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Engineer II

Job ID: 25-09454

Title:  Engineer II Protein Purification
Duration: 12 Months
Location: Elkton, VA
Education Minimum Requirement:
A bachelor's (w/4+ yrs. purification experience) or masters (2+ yrs purification experience) or Ph.D. degree (direct experience) in a relevant scientific discipline (biochemistry, chemistry, biology), life sciences, Engineering

Required Experience and Skills
• Experience with protein purification from bacterial or yeast or mammalian systems in a laboratory or pilot or commercial scale.
• Hands on experience using AKTA Purification Systems
• Strong background in protein biochemistry
• Experience with biochemical and analytical methods for protein and or DNA analysis.

• Wet chemistry laboratory skills (pipetting, buffer preparation, filtration, dilution, stoichiometry)
• Proficiency with technical writing and laboratory records (laboratory notebooks, chemical inventory, data management)
• Excellent collaborative and team-working skills
• Strong technical problem-solving abilities, data interpretation and presentation competence
Preferred Experience and Skills:
• Experience working with protein purification
• Experience working with column chromatography and AKTA/Unicorn systems
• Experience working with tangential flow filtration processing – microfiltration/ultrafiltration
• Experience performing fermentation or cell culture studies at the labs scale
• Experience performing fermentation or cell culture studies using shake flasks
• Knowledge of yeast fermentation and metabolism
• Experience working in GMP and GLP environments
• Experience with laboratory operations
• Experience with facility operations
• Experience working in an industry environments
• Experience conducting laboratory scale process characterization studies
• Experience troubleshooting technical issues (experimental, equipment-related issues)
• Experience with material characterization
• Experience working with enzymes and peptides
• Experience in method development and method transfer
• Experience with six sigma, 5S principles

Responsibilities:
As a member of Process Development, the primary goal of the position is to:
• Characterize proteins, VLPs and constituent components with a variety of analytical techniques
• Participate in/drive fermentation and/or purification of proteins/VLPs at lab and pilot scale
• Provide support for execution of process development activities and manufacturing investigations
• Perform laboratory scale studies
• Design and troubleshoot experiments
• Employ excellent verbal and written communication skills
• Author laboratory notebooks and technical documents
• Work both independently and within multidisciplinary team
• Produce high quality work against firm deadlines
• Maintain good sample handling practices
• Balance and prioritize competing deliverables

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